The ProMTag UPECK Multi kit will be available for purchase soon. If you are interested in beta testing the kit, contact us at info@impactproteomics.com
The ProMTag UPECK Multi kit produces high yield, reproducible RNA, DNA, and peptide samples from the same starting sample for analysis. RNA and DNA are high quality and compatible with downstream approaches such as RNA-Seq and whole genome sequencing. Peptides are in a volatile, mass spectrometry compatible buffer for proteomics analysis. Included reagents and an easy-to-perform workflow take you from cell lysis all the way to RNA, DNA, and peptides.
Each kit includes:
- Strong lysis buffer
- ProMTag protein tagging agent
- ProMTag protein capture resin
- Wash buffers
- DNA elution buffer
- Reducing and alkylating reagents
- Stabilized MT-Trypsin
- Volatile, mass spectrometry compatible elution buffer
- Low protein binding tubes
- Waste collection tubes
What you’ll need:
- Tabletop centrifuge
- Rotisserie or shaker
- Heating block
- RNase and/or DNase depending on desired downstream applications
How it works:
Our patent pending reversible chemistry allows for extraction, cleanup, and digestion in an fast, easy-to-perform workflow:
- Lyse cells or tissue with lysis buffer
- Label proteins with ProMTag
- Capture RNA, DNA, and proteins on ProMTag capture resin
- Wash away contaminants
- Release RNA and DNA from ProMTag capture resin
- Release proteins from ProMTag capture resin
- Digest proteins into peptides with stabilized MT-Trypsin
- Capture MT-Trypsin on ProMTag capture resin
- Elute clean, digested peptides ready for mass spectrometry
Do you want to customize your kit for your needs? Question about the workflow? Reach out to us at info@impactproteomics.com
