Universal Protein Extraction and Cleanup Kit – Peptides (UPECK-Pep)

From: $250.00

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SKU: Pep-1000 Category:

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The UPECK-Pep kit produces high-quality peptide samples for mass spectrometry analysis in a fast, easy-to-do workflow from cell lysis all the way to peptides.  Works with any starting sample for fast,  high yield extraction, cleanup of protein samples, and digestion into peptides for mass spectrometry analysis.

Each kit includes:

  • Strong lysis buffer
  • ProMTag protein tagging agent
  • ProMTag protein capture resin
  • Wash buffers
  • Reducing and alkylating reagents
  • Stabilized mP-Trypsin
  • Mass spectrometry compatible and volatile elution buffer
  • Low protein binding tubes
  • Waste collection tubes

What you’ll need:

  • Tabletop centrifuge
  • Rotisserie or shaker
  • Heating block

How it works:

Our patent pending reversible chemistry allows for extraction, cleanup, and digestion in an fast, easy-to-perform workflow:

  1. Lyse cells or tissue with lysis buffer
  2. Label proteins with ProMTag
  3. Capture proteins with ProMTag resin
  4. Wash away contaminants
  5. Release proteins from ProMTag resin
  6. Reduce/alkylate proteins
  7. Digest proteins into peptide with stabilized ProMTag trypsin
  8. Capture ProMTag trypsin on ProMTag resin
  9. Elute clean, digested peptides

See more on our How It Works page

Starting protein amount per prep

5 – 40 ug, 41 – 200 ug

Starting protein concentration per prep

less than 1 mg/mL, 1 mg/mL or greater