Purify target antigens ready for mass spectrometry without worrying about contaminating antibodies, detergents, or salts. One easy workflow for the capture, cleanup, and release of mass spec ready antigens.
The ProMTag Immunoprecipitation to Mass Spectrometry (IP-to-MS) Kit kit produces high-quality antigen samples for mass spectrometry analysis in a fast, easy-to-do workflow from immobilization of antibodies on Protein A resin all the way to antigen peptides ready for analysis. Each kit contains reagents for 8 immunoprecipitations.
Download the ProMTag IP-to-MS Kit User Manual
Each kit includes:
- Lysis buffer
- Protein A resin for immobilization of antibodies for immunoprecipitation
- ProMTag protein tagging agent
- ProMTag protein capture resin
- Wash buffers
- Stabilized MT-Trypsin
- Mass spectrometry compatible and volatile elution buffer
- Resin capture (RC) tubes
- Low protein binding tubes
- Waste collection tubes
What you’ll need:
- Protease inhibitors
- Cell lysate, tissue lysate, or other protein source
- Serum, plasma, or other antibody source
- Tabletop centrifuge
- Rotisserie or shaker
- Heating block
