For the best results, start with the best sample! Our universal protein extraction and cleanup kit for whole proteins works with any starting sample for fast, high yield cleanup. This kit has been optimized for two dimensional gel electrophoresis, but can also be used to remove contaminating small molecules/non-protein biomolecules from a whole protein sample to be analyzed. The included elution buffer can be customized for the appropriate application by contacting us at info@impactproteomics.com prior to placing your order
Kit includes:
Strong lysis buffer
ProMTag protein tagging agent
ProMTag protein capture resin
Wash buffers
Elution buffer
Low protein binding tubes
Waste collection tubes
What you’ll need:
Tabletop centrifuge
Rotisserie or shaker
How it works:
Our patent pending reversible chemistry allows taggin, capture, cleanup, and elution of proteins in a fast, easy-to-do workflow:
- Lyse cells or tissue with the strong lysis buffer provided
- Label proteins with ProMTag
- Capture protein with ProMTag capture resin
- Wash away contaminants
- Elute clean protein sample
See more on our How It Works page
Download the full length protocol here
Download the SDS sheets for the components here
