Universal Protein Extraction and Cleanup Kit – Whole proteins (UPECK-WP)

$150.00$225.00

Universal protein extraction and cleanup kit of whole proteins for gel electrophoresis.  This kit has been optimized for two dimensional gel electrophoresis, but can also be used to remove contaminating small molecules/non-protein biomolecules from a whole protein sample to be analyzed.

 

Kit includes:

Strong lysis buffer

ProMTag protein tagging agent

ProMTag protein capture resin

Wash buffers

Elution buffer

Low protein binding tubes

Waste collection tubes

Clear
SKU: WP-2000 Category:

For the best results, start with the best sample!  Our universal protein extraction and cleanup kit for whole proteins works with any starting sample for fast, high yield cleanup.  This kit has been optimized for two dimensional gel electrophoresis, but can also be used to remove contaminating small molecules/non-protein biomolecules from a whole protein sample to be analyzed.  The included elution buffer can be customized for the appropriate application by contacting us at info@impactproteomics.com prior to placing your order

 

Kit includes:

Strong lysis buffer

ProMTag protein tagging agent

ProMTag protein capture resin

Wash buffers

Elution buffer

Low protein binding tubes

Waste collection tubes

 

What you’ll need:

Tabletop centrifuge

Rotisserie or shaker

 

How it works:

 

Our patent pending reversible chemistry allows taggin, capture, cleanup, and elution of proteins in a fast, easy-to-do workflow:

  1. Lyse cells or tissue with the strong lysis buffer provided
  2. Label proteins with ProMTag
  3. Capture protein with ProMTag capture resin
  4. Wash away contaminants
  5. Elute clean protein sample

 

See more on our How It Works page

 

Download the full length protocol here

Download the SDS sheets for the components here

Amount of starting protein per prep

1-40 ug, 41- 200 ug, >200 ug

Starting protein concentration

Less than 1 mg/mL, 1 mg/mL or greater

Elution buffer

Two-dimensional gel electrophoresis compatible, SDS gel electrophoresis compatible, Mass spectrometry compatible, Other